Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11009 and 11010 are separated by 523 nucleotides11010 and 11011 are separated by 266 nucleotides11011 and 11012 are separated by 169 nucleotides11012 and 11013 are separated by 325 nucleotides mRNA_2641: 11009 - Hypothetical Protein, at 658,203 to 659,346 11009 mRNA_2642: 11010 - K17428 MRPL47, NCM1 large subunit ribosomal protein L47, at 659,870 to 660,906 11010 mRNA_2643: 11011 - K01254 LTA4H leukotriene-A4 hydrolase, at 661,173 to 664,262 11011 mRNA_2644: 11012 - SUPERFAMILY--SSF52058, at 664,432 to 665,418 11012 mRNA_2645: 11013 - Hypothetical Protein, at 665,744 to 666,738 11013
Group Condition mRNA_2641 mRNA_2642 mRNA_2643 mRNA_2644 mRNA_2645
11009 11010 11011 11012 11013
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. -0.4 -1.6 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 N.D. -0.2 -0.8 +0.0
auxotrophy Dropout Complete +0.5 N.D. -0.1 -0.8 -0.7
growth on oleic acid YNB Oleic Acid +0.4 N.D. -0.3 -0.7 -0.4
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.2 -1.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. +0.1 -0.7 -0.9
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.3 -0.9 -0.5
auxotrophy YNB Glucose +0.2 N.D. +0.3 -0.9 -0.2
growth on oleic acid YNB Glucose +0.2 N.D. +0.0 -0.7 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 N.D. -0.5 -0.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.0 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.1 +0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.3 -0.4 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.1 +0.4 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.1 +0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.0 +0.7 -0.4
growth on ypd YPD +0.1 N.D. -0.1 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.1 +0.8 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.4 N.D. -0.4 +0.4 +0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.3 +0.5 +0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 N.D. +0.1 +1.0 +0.5
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