Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11046 and 11047 are separated by 71 nucleotides11047 and 11048 are separated by 495 nucleotides11048 and 11049 are separated by 710 nucleotides11049 and 11050 are separated by 996 nucleotides mRNA_2678: 11046 - KOG3102 Uncharacterized conserved protein, at 758,219 to 759,317 11046 mRNA_2679: 11047 - K14262 DUG2 di- and tripeptidase, at 759,389 to 763,569 11047 mRNA_2680: 11048 - Hypothetical Protein, at 764,065 to 765,232 11048 mRNA_2681: 11049 - Hypothetical Protein, at 765,943 to 766,697 11049 mRNA_2682: 11050 - K00589 MET1 uroporphyrin-III C-methyltransferase, at 767,694 to 769,770 11050
Group Condition mRNA_2678 mRNA_2679 mRNA_2680 mRNA_2681 mRNA_2682
11046 11047 11048 11049 11050
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 +0.1 -0.6 -0.3 -2.0
growth on ricinoleic acid YNB Glucose +0.0 +0.2 +0.2 +0.2 -3.7
auxotrophy YNB Glucose +0.2 +0.0 -0.3 +0.1 -2.8
growth on ricinoleic acid YNB Ricinoleic Acid -2.0 -0.2 +0.7 +0.0 -1.3
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 -0.1 +0.2 -2.2
growth on oleic acid YNB Glucose -0.2 +0.0 +0.0 +0.3 -2.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.1 +0.3 -0.4 -1.5
ypd buoyancy 40 hrs No Buoyancy Separation -1.7 -0.1 +0.2 +0.4 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.2 +0.0 +0.4 -2.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 +0.1 +0.0 -0.9
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 -0.1 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.3 -0.2 +0.8 -1.5
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.3 +0.0 +0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.6 +0.1 +0.3 +0.4 -0.3
auxotrophy Dropout Complete +0.6 -0.3 -0.1 -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.5 -0.0 -0.4 +0.1 +0.1
growth on ypd YPD +0.3 -0.1 +0.2 +0.5 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 +0.0 +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.6 -0.0 -0.0 +0.1 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.3 +0.0 +0.8 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.2 -0.3 +0.1 +1.7
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