Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11047 and 11048 are separated by 495 nucleotides11048 and 11049 are separated by 710 nucleotides11049 and 11050 are separated by 996 nucleotides11050 and 11051 are separated by 175 nucleotides mRNA_2679: 11047 - K14262 DUG2 di- and tripeptidase, at 759,389 to 763,569 11047 mRNA_2680: 11048 - Hypothetical Protein, at 764,065 to 765,232 11048 mRNA_2681: 11049 - Hypothetical Protein, at 765,943 to 766,697 11049 mRNA_2682: 11050 - K00589 MET1 uroporphyrin-III C-methyltransferase, at 767,694 to 769,770 11050 mRNA_2683: 11051 - K07300 chaA, CAX Ca2+-H+ antiporter, at 769,946 to 774,939 11051
Group Condition mRNA_2679 mRNA_2680 mRNA_2681 mRNA_2682 mRNA_2683
11047 11048 11049 11050 11051
growth on ricinoleic acid YNB Glucose +0.2 +0.2 +0.2 -3.7 -0.2
auxotrophy YNB Glucose +0.0 -0.3 +0.1 -2.8 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.6 -0.3 -2.0 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.1 +0.2 -2.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.3 -0.4 -1.5 -0.3
growth on oleic acid YNB Glucose +0.0 +0.0 +0.3 -2.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 +0.4 -2.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.7 +0.0 -1.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 +0.0 -0.9 -0.1
auxotrophy Dropout Complete -0.3 -0.1 -0.2 -0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.4 +0.1 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.1 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.2 +0.8 -1.5 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 +0.3 +0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.0 +0.2 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.2 +0.4 -0.4 +0.2
growth on ypd YPD -0.1 +0.2 +0.5 -0.1 -0.0
growth on oleic acid YNB Oleic Acid +0.1 +0.3 +0.4 -0.3 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.0 +0.1 +0.5 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.0 +0.8 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.3 +0.1 +1.7 +0.2
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