Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11049 and 11050 are separated by 996 nucleotides11050 and 11051 are separated by 175 nucleotides11051 and 11052 are separated by 704 nucleotides11052 and 11053 are separated by 762 nucleotides mRNA_2681: 11049 - Hypothetical Protein, at 765,943 to 766,697 11049 mRNA_2682: 11050 - K00589 MET1 uroporphyrin-III C-methyltransferase, at 767,694 to 769,770 11050 mRNA_2683: 11051 - K07300 chaA, CAX Ca2+-H+ antiporter, at 769,946 to 774,939 11051 mRNA_2684: 11052 - K02144 ATPeV1H V-type H+-transporting ATPase subunit H, at 775,644 to 777,796 11052 mRNA_2685: 11053 - Hypothetical Protein, at 778,559 to 779,074 11053
Group Condition mRNA_2681 mRNA_2682 mRNA_2683 mRNA_2684 mRNA_2685
11049 11050 11051 11052 11053
growth on ricinoleic acid YNB Glucose +0.2 -3.7 -0.2 N.D. -0.4
auxotrophy YNB Glucose +0.1 -2.8 -0.1 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -2.0 -0.3 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -1.5 -0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 -2.2 +0.1 N.D. -0.2
growth on oleic acid YNB Glucose +0.3 -2.1 +0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -1.3 -0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -2.1 -0.1 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.9 -0.1 N.D. -0.1
auxotrophy Dropout Complete -0.2 -0.0 -0.1 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 -0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 -1.5 +0.3 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.4 +0.2 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.2 -0.2 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 -0.1 N.D. -0.0
growth on oleic acid YNB Oleic Acid +0.4 -0.3 +0.0 N.D. -0.0
growth on ypd YPD +0.5 -0.1 -0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.5 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 +0.1 +0.3 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +1.7 +0.2 N.D. +0.2
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