Fitness for 4 genes in Rhodosporidium toruloides IFO0880

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500 nt 11050 and 11051 are separated by 175 nucleotides11051 and 11052 are separated by 704 nucleotides11052 and 11053 are separated by 762 nucleotides mRNA_2682: 11050 - K00589 MET1 uroporphyrin-III C-methyltransferase, at 767,694 to 769,770 11050 mRNA_2683: 11051 - K07300 chaA, CAX Ca2+-H+ antiporter, at 769,946 to 774,939 11051 mRNA_2684: 11052 - K02144 ATPeV1H V-type H+-transporting ATPase subunit H, at 775,644 to 777,796 11052 mRNA_2685: 11053 - Hypothetical Protein, at 778,559 to 779,074 11053
Group Condition mRNA_2682 mRNA_2683 mRNA_2684 mRNA_2685
11050 11051 11052 11053
growth on ricinoleic acid YNB Glucose -3.7 -0.2 N.D. -0.4
auxotrophy YNB Glucose -2.8 -0.1 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -2.0 -0.3 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine -2.2 +0.1 N.D. -0.2
growth on oleic acid YNB Glucose -2.1 +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.1 -0.1 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate -1.5 -0.3 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 -0.2 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.5 +0.3 N.D. +0.3
auxotrophy Dropout Complete -0.0 -0.1 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.2 N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.3 +0.0 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.2 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.1 N.D. +0.0
growth on ypd YPD -0.1 -0.0 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.7 +0.2 N.D. +0.2
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