Fitness for 3 genes in Rhodosporidium toruloides IFO0880

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500 nt 11051 and 11052 are separated by 704 nucleotides11052 and 11053 are separated by 762 nucleotides mRNA_2683: 11051 - K07300 chaA, CAX Ca2+-H+ antiporter, at 769,946 to 774,939 11051 mRNA_2684: 11052 - K02144 ATPeV1H V-type H+-transporting ATPase subunit H, at 775,644 to 777,796 11052 mRNA_2685: 11053 - Hypothetical Protein, at 778,559 to 779,074 11053
Group Condition mRNA_2683 mRNA_2684 mRNA_2685
11051 11052 11053
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.4
auxotrophy Dropout Complete -0.1 N.D. -0.5
auxotrophy YNB Glucose -0.1 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.0
growth on oleic acid YNB Glucose +0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.2
growth on ypd YPD -0.0 N.D. -0.0
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. +0.4
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