Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11098 and 11099 are separated by 10 nucleotides11099 and 11100 are separated by 1517 nucleotides11100 and 11101 are separated by 209 nucleotides11101 and 11102 are separated by 292 nucleotides mRNA_2730: 11098 - Hypothetical Protein, at 92,250 to 93,018 11098 mRNA_2731: 11099 - HMMPfam-Domain of unknown function (DUF4112)-PF13430, at 93,029 to 93,911 11099 mRNA_2732: 11100 - Hypothetical Protein, at 95,429 to 95,692 11100 mRNA_2733: 11101 - KOG0254 Predicted transporter (major facilitator superfamily), at 95,902 to 98,475 11101 mRNA_2734: 11102 - K14779 DDX52, ROK1 ATP-dependent RNA helicase DDX52/ROK1, at 98,768 to 101,128 11102
Group Condition mRNA_2730 mRNA_2731 mRNA_2732 mRNA_2733 mRNA_2734
11098 11099 11100 11101 11102
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.2 -0.2 N.D.
auxotrophy Dropout Complete +0.0 N.D. -0.1 -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. +0.0 -0.0 N.D.
growth on ypd YPD +0.2 N.D. -0.2 +0.0 N.D.
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.2 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.0 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.2 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.1 -0.1 N.D.
auxotrophy YNB Glucose -0.1 N.D. +0.2 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. +0.2 -0.1 N.D.
growth on oleic acid YNB Glucose -0.0 N.D. +0.3 -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.2 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.2 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.2 +0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 N.D. +0.1 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.4 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. +0.5 +0.2 N.D.
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