Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11099 and 11100 are separated by 1517 nucleotides11100 and 11101 are separated by 209 nucleotides11101 and 11102 are separated by 292 nucleotides11102 and 11103 are separated by 133 nucleotides mRNA_2731: 11099 - HMMPfam-Domain of unknown function (DUF4112)-PF13430, at 93,029 to 93,911 11099 mRNA_2732: 11100 - Hypothetical Protein, at 95,429 to 95,692 11100 mRNA_2733: 11101 - KOG0254 Predicted transporter (major facilitator superfamily), at 95,902 to 98,475 11101 mRNA_2734: 11102 - K14779 DDX52, ROK1 ATP-dependent RNA helicase DDX52/ROK1, at 98,768 to 101,128 11102 mRNA_2735: 11103 - K02257 COX10 protoheme IX farnesyltransferase, at 101,262 to 102,881 11103
Group Condition mRNA_2731 mRNA_2732 mRNA_2733 mRNA_2734 mRNA_2735
11099 11100 11101 11102 11103
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.2 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.1 -0.2 N.D. N.D.
growth on ypd YPD N.D. -0.2 +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.2 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.3 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.1 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.3 -0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.1 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.2 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.0 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.2 N.D. N.D.
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