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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11101 and 11102 are separated by 292 nucleotides
11102 and 11103 are separated by 133 nucleotides
11103 and 11104 are separated by 185 nucleotides
11104 and 11105 are separated by 280 nucleotides
mRNA_2733: 11101 - KOG0254 Predicted transporter (major facilitator superfamily), at 95,902 to 98,475
11101
mRNA_2734: 11102 - K14779 DDX52, ROK1 ATP-dependent RNA helicase DDX52/ROK1, at 98,768 to 101,128
11102
mRNA_2735: 11103 - K02257 COX10 protoheme IX farnesyltransferase, at 101,262 to 102,881
11103
mRNA_2736: 11104 - K11093 SNRP70 U1 small nuclear ribonucleoprotein 70kDa, at 103,067 to 104,847
11104
mRNA_2737: 11105 - K09584 PDIA6, TXNDC7 protein disulfide-isomerase A6, at 105,128 to 106,332
11105
Group
Condition
mRNA
_2733
mRNA
_2734
mRNA
_2735
mRNA
_2736
mRNA
_2737
11101
11102
11103
11104
11105
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.2
N.D.
N.D.
N.D.
-0.9
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
N.D.
N.D.
N.D.
-0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
N.D.
N.D.
N.D.
-0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.0
N.D.
N.D.
N.D.
-0.5
growth on ricinoleic acid
YNB Glucose
-0.0
N.D.
N.D.
N.D.
-0.3
auxotrophy
Dropout Complete
-0.2
N.D.
N.D.
N.D.
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.2
N.D.
N.D.
N.D.
-0.0
ypd buoyancy
40 hrs High Buoyancy
-0.0
N.D.
N.D.
N.D.
-0.1
growth on oleic acid
YNB Glucose
-0.0
N.D.
N.D.
N.D.
-0.1
auxotrophy
YNB Glucose 75mM Arginine
-0.1
N.D.
N.D.
N.D.
-0.1
growth on oleic acid
YNB Oleic Acid
+0.1
N.D.
N.D.
N.D.
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.0
N.D.
N.D.
N.D.
-0.1
growth on ypd
YPD
+0.0
N.D.
N.D.
N.D.
-0.1
auxotrophy
YNB Glucose
+0.1
N.D.
N.D.
N.D.
-0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.1
N.D.
N.D.
N.D.
+0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.1
N.D.
N.D.
N.D.
+0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.0
N.D.
N.D.
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.0
N.D.
N.D.
N.D.
+0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
N.D.
N.D.
N.D.
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
N.D.
N.D.
N.D.
+0.4
ypd buoyancy
40 hrs Low Buoyancy
-0.1
N.D.
N.D.
N.D.
+0.6
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