Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11111 and 11112 are separated by 431 nucleotides11112 and 11113 are separated by 949 nucleotides11113 and 11114 are separated by 236 nucleotides11114 and 11115 are separated by 468 nucleotides mRNA_2743: 11111 - KOG4491 Predicted membrane protein, at 119,009 to 120,330 11111 mRNA_2744: 11112 - K20798 HENMT1 small RNA 2'-O-methyltransferase, at 120,762 to 122,799 11112 mRNA_2745: 11113 - HMMPfam-GlcNAc-PI de-N-acetylase-PF02585,SUPERFAMILY--SSF102588,SUPERFAMILY--SSF49785, at 123,749 to 127,463 11113 mRNA_2746: 11114 - K00859 coaE dephospho-CoA kinase, at 127,700 to 128,562 11114 mRNA_2747: 11115 - Hypothetical Protein, at 129,031 to 129,720 11115
Group Condition mRNA_2743 mRNA_2744 mRNA_2745 mRNA_2746 mRNA_2747
11111 11112 11113 11114 11115
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 -0.2 N.D. -0.4
auxotrophy YNB Glucose -0.3 -0.4 -0.1 N.D. +0.3
growth on ypd YPD -0.0 -0.1 -0.1 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.3 -0.2 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.3 -0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.3 -0.2 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 -0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.1 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.2 -0.2 N.D. +0.4
growth on oleic acid YNB Glucose -0.2 -0.1 +0.1 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.0 +0.1 N.D. +0.1
auxotrophy Dropout Complete +0.1 -0.2 -0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.1 -0.1 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.0 -0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 -0.2 N.D. +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.6 +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.1 -0.0 N.D. +0.4
growth on oleic acid YNB Oleic Acid +0.0 +0.1 +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.3 +0.0 N.D. +0.4
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