Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11126 and 11127 are separated by 262 nucleotides11127 and 11128 are separated by 414 nucleotides11128 and 11129 are separated by 665 nucleotides11129 and 11130 are separated by 231 nucleotides mRNA_2758: 11126 - Hypothetical Protein, at 150,871 to 152,585 11126 mRNA_2759: 11127 - K12158 NEDD8 ubiquitin-like protein Nedd8, at 152,848 to 153,537 11127 mRNA_2760: 11128 - HMMPfam-Acyltransferase-PF01553,SMART-Phosphate acyltransferases-SM00563,SUPERFAMILY--SSF69593, at 153,952 to 156,416 11128 mRNA_2761: 11129 - K00031 IDH1, IDH2, icd isocitrate dehydrogenase, at 157,082 to 158,929 11129 mRNA_2762: 11130 - BLAST Protein pof8 [Termitomyces sp. J132], at 159,161 to 161,620 11130
Group Condition mRNA_2758 mRNA_2759 mRNA_2760 mRNA_2761 mRNA_2762
11126 11127 11128 11129 11130
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.3 -1.8 +0.0
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.3 -1.2 -0.3
auxotrophy YNB Glucose -0.1 N.D. +0.3 -1.7 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.2 -1.8 +0.2
growth on oleic acid YNB Glucose +0.0 N.D. +0.3 -1.4 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -0.0 -0.6 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.1 -0.9 +0.1
auxotrophy Dropout Complete -0.1 N.D. -0.2 -0.5 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.0 -0.9 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. +0.4 -0.8 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.3 -0.9 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.0 -0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.3 -0.3 +0.3
growth on ypd YPD +0.7 N.D. -0.6 -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.2 +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. +0.1 +0.3 +0.2
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.1 +0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.2 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.3 -0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. -0.1 +0.8 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. +0.2 +0.3 +0.1
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