Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11129 and 11130 are separated by 231 nucleotides11130 and 11131 are separated by 292 nucleotides11131 and 11132 are separated by 437 nucleotides11132 and 11133 are separated by 269 nucleotides mRNA_2761: 11129 - K00031 IDH1, IDH2, icd isocitrate dehydrogenase, at 157,082 to 158,929 11129 mRNA_2762: 11130 - BLAST Protein pof8 [Termitomyces sp. J132], at 159,161 to 161,620 11130 mRNA_2763: 11131 - Hypothetical Protein, at 161,913 to 163,067 11131 mRNA_2764: 11132 - Hypothetical Protein, at 163,505 to 164,257 11132 mRNA_2765: 11133 - Hypothetical Protein, at 164,527 to 165,110 11133
Group Condition mRNA_2761 mRNA_2762 mRNA_2763 mRNA_2764 mRNA_2765
11129 11130 11131 11132 11133
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -0.3 +0.0 -0.6 -2.4
auxotrophy YNB Glucose 75mM Methionine -1.8 +0.2 -0.3 -0.0 -1.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 +0.2 +0.4 -0.0 -3.1
auxotrophy Dropout Complete -0.5 +0.2 -0.3 +0.0 -1.9
auxotrophy YNB Glucose -1.7 -0.1 -0.4 +0.3 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.8 -0.0 -0.5 -0.3 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.8 +0.0 -0.1 -0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.1 +0.2 -0.3 -1.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.1 -1.0 +0.6 -1.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 -0.1 -0.2 -1.3
facs on lipid accumulation Sorted High BODIPY Gate -0.9 +0.1 +0.5 +0.4 -1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.9 +0.1 -0.0 +0.4 -0.5
growth on ricinoleic acid YNB Glucose -1.2 -0.3 -0.4 +1.1 -0.2
growth on oleic acid YNB Glucose -1.4 +0.0 +0.1 -0.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 -0.5 +0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.3 +0.1 -0.1 -0.7
growth on ypd YPD -0.1 -0.0 +0.2 -0.0 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.0 -0.0 +0.0 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.8 -0.3 -0.8 +0.6 -0.7
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 +0.0 -0.2 -0.4
growth on oleic acid YNB Oleic Acid +0.3 -0.0 -0.2 +0.5 +0.5
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