Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11133 and 11134 are separated by 250 nucleotides11134 and 11135 are separated by 130 nucleotides11135 and 11136 are separated by 145 nucleotides11136 and 11137 are separated by 223 nucleotides mRNA_2765: 11133 - Hypothetical Protein, at 164,527 to 165,110 11133 mRNA_2766: 11134 - KOG2827 Uncharacterized conserved protein, at 165,361 to 166,420 11134 mRNA_2767: 11135 - K10084 EDEM1 ER degradation enhancer, mannosidase alpha-like 1, at 166,551 to 170,333 11135 mRNA_2768: 11136 - K08681 pdxT, pdx2 5'-phosphate synthase pdxT subunit, at 170,479 to 171,341 11136 mRNA_2769: 11137 - K06215 pdxS, pdx1 pyridoxal 5'-phosphate synthase pdxS subunit, at 171,565 to 173,167 11137
Group Condition mRNA_2765 mRNA_2766 mRNA_2767 mRNA_2768 mRNA_2769
11133 11134 11135 11136 11137
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -3.1 N.D. +0.0 -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine -1.7 N.D. -0.0 -0.5 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -1.7 N.D. -0.5 +0.1 -0.3
auxotrophy Dropout Complete -1.9 N.D. +0.0 -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -2.4 N.D. +0.2 +0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. -0.3 -0.4 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -1.0 N.D. -0.1 -0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy -1.3 N.D. +0.1 +0.0 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -1.1 N.D. +0.2 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.3 -0.2 +0.1
growth on ypd YPD -0.6 N.D. -0.3 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 N.D. +0.0 +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 N.D. +0.4 -0.0 -0.3
auxotrophy YNB Glucose -0.4 N.D. +0.0 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. +0.3 -0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. -0.2 +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.3 -0.0 +0.1
growth on oleic acid YNB Glucose +0.5 N.D. +0.0 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. +0.1 +0.4 +0.0
growth on oleic acid YNB Oleic Acid +0.5 N.D. -0.0 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.2 +0.0 +0.1
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