Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11140 and 11141 are separated by 521 nucleotides11141 and 11142 are separated by 1049 nucleotides11142 and 11143 are separated by 369 nucleotides11143 and 11144 are separated by 58 nucleotides mRNA_2772: 11140 - SUPERFAMILY--SSF56112, at 177,192 to 178,295 11140 mRNA_2773: 11141 - K02953 RP-S13e, RPS13 small subunit ribosomal protein S13e, at 178,817 to 179,625 11141 mRNA_2774: 11142 - K02991 RP-S6e, RPS6 small subunit ribosomal protein S6e, at 180,675 to 181,907 11142 mRNA_2775: 11143 - KOG1646 40S ribosomal protein S6, at 182,277 to 183,930 11143 mRNA_2776: 11144 - K14292 TGS1 trimethylguanosine synthase, at 183,989 to 185,404 11144
Group Condition mRNA_2772 mRNA_2773 mRNA_2774 mRNA_2775 mRNA_2776
11140 11141 11142 11143 11144
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. N.D. -0.2 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. N.D. -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. N.D. +0.5 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. +0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. N.D. -0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. -0.2 +0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. -0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. +0.0 +0.2
growth on ypd YPD +0.2 N.D. N.D. +0.4 -0.4
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. +0.0 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. N.D. -0.2 +0.1
auxotrophy YNB Glucose +0.5 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. -0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.3 +0.1
growth on oleic acid YNB Glucose +0.5 N.D. N.D. -0.2 +0.3
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