Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11142 and 11143 are separated by 369 nucleotides11143 and 11144 are separated by 58 nucleotides11144 and 11145 are separated by 645 nucleotides11145 and 11146 are separated by 1237 nucleotides mRNA_2774: 11142 - K02991 RP-S6e, RPS6 small subunit ribosomal protein S6e, at 180,675 to 181,907 11142 mRNA_2775: 11143 - KOG1646 40S ribosomal protein S6, at 182,277 to 183,930 11143 mRNA_2776: 11144 - K14292 TGS1 trimethylguanosine synthase, at 183,989 to 185,404 11144 mRNA_2777: 11145 - K00948 PRPS, prsA ribose-phosphate pyrophosphokinase, at 186,050 to 187,746 11145 mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295 11146
Group Condition mRNA_2774 mRNA_2775 mRNA_2776 mRNA_2777 mRNA_2778
11142 11143 11144 11145 11146
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.5 -0.3 N.D. -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.4 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 +0.0 N.D. -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.1 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 -0.2 N.D. -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 N.D. +0.1
growth on ypd YPD N.D. +0.4 -0.4 N.D. -0.2
auxotrophy Dropout Complete N.D. -0.3 +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.1 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 -0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.2 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 -0.3 N.D. +0.5
auxotrophy YNB Glucose N.D. -0.1 -0.1 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 +0.1 N.D. +0.3
growth on oleic acid YNB Glucose N.D. -0.2 +0.3 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.3 N.D. +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.0 N.D. +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 +0.1 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 +0.3 N.D. +0.7
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