Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11143 and 11144 are separated by 58 nucleotides11144 and 11145 are separated by 645 nucleotides11145 and 11146 are separated by 1237 nucleotides11146 and 11147 are separated by 129 nucleotides mRNA_2775: 11143 - KOG1646 40S ribosomal protein S6, at 182,277 to 183,930 11143 mRNA_2776: 11144 - K14292 TGS1 trimethylguanosine synthase, at 183,989 to 185,404 11144 mRNA_2777: 11145 - K00948 PRPS, prsA ribose-phosphate pyrophosphokinase, at 186,050 to 187,746 11145 mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295 11146 mRNA_2779: 11147 - K18278 THI5 pyrimidine precursor biosynthesis enzyme, at 191,425 to 193,018 11147
Group Condition mRNA_2775 mRNA_2776 mRNA_2777 mRNA_2778 mRNA_2779
11143 11144 11145 11146 11147
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -0.3 N.D. -1.3 -0.8
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.0 N.D. -0.8 -0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.2 N.D. -0.3 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.1 N.D. -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.4 N.D. -0.2 +0.1
growth on ypd YPD +0.4 -0.4 N.D. -0.2 -0.4
auxotrophy YNB Glucose -0.1 -0.1 N.D. +0.4 -0.5
auxotrophy Dropout Complete -0.3 +0.1 N.D. +0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.1 N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.1 N.D. +0.3 -0.5
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.1 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.1 N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 N.D. +0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. +0.7 -1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.2 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.3 N.D. +0.5 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.3 N.D. +0.4 -0.2
growth on oleic acid YNB Glucose -0.2 +0.3 N.D. +0.4 +0.0
growth on ricinoleic acid YNB Glucose +0.0 +0.0 N.D. +0.5 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.1 N.D. +0.3 +0.1
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