Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11144 and 11145 are separated by 645 nucleotides11145 and 11146 are separated by 1237 nucleotides11146 and 11147 are separated by 129 nucleotides11147 and 11148 are separated by 1083 nucleotides mRNA_2776: 11144 - K14292 TGS1 trimethylguanosine synthase, at 183,989 to 185,404 11144 mRNA_2777: 11145 - K00948 PRPS, prsA ribose-phosphate pyrophosphokinase, at 186,050 to 187,746 11145 mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295 11146 mRNA_2779: 11147 - K18278 THI5 pyrimidine precursor biosynthesis enzyme, at 191,425 to 193,018 11147 mRNA_2780: 11148 - KOG0546 HSP90 co-chaperone CPR7/Cyclophilin, at 194,102 to 194,966 11148
Group Condition mRNA_2776 mRNA_2777 mRNA_2778 mRNA_2779 mRNA_2780
11144 11145 11146 11147 11148
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -1.3 -0.8 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. -0.8 -0.7 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.3 -0.5 -0.3
growth on ypd YPD -0.4 N.D. -0.2 -0.4 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.4 -0.2 +0.2
auxotrophy YNB Glucose -0.1 N.D. +0.4 -0.5 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.2 +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.3 +0.1 -0.6
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.1 +0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.2 -0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.7 -1.0 -0.0
auxotrophy Dropout Complete +0.1 N.D. +0.0 -0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. +0.5 +0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.2 -0.1 +0.0
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.5 -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.3 -0.5 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.1 +0.2 +0.1
growth on oleic acid YNB Glucose +0.3 N.D. +0.4 +0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.3 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.1 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.4 -0.2 +0.4
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