Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11145 and 11146 are separated by 1237 nucleotides11146 and 11147 are separated by 129 nucleotides11147 and 11148 are separated by 1083 nucleotides11148 and 11149 are separated by 173 nucleotides mRNA_2777: 11145 - K00948 PRPS, prsA ribose-phosphate pyrophosphokinase, at 186,050 to 187,746 11145 mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295 11146 mRNA_2779: 11147 - K18278 THI5 pyrimidine precursor biosynthesis enzyme, at 191,425 to 193,018 11147 mRNA_2780: 11148 - KOG0546 HSP90 co-chaperone CPR7/Cyclophilin, at 194,102 to 194,966 11148 mRNA_2781: 11149 - K09506 DNAJA5 DnaJ homolog subfamily A member 5, at 195,140 to 197,521 11149
Group Condition mRNA_2777 mRNA_2778 mRNA_2779 mRNA_2780 mRNA_2781
11145 11146 11147 11148 11149
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.3 -0.8 -0.4 -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.8 -0.7 -0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.5 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.2 +0.2 -0.2
growth on ypd YPD N.D. -0.2 -0.4 +0.0 -0.1
auxotrophy Dropout Complete N.D. +0.0 -0.2 +0.0 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.3 -0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.1 -0.6 -0.2
auxotrophy YNB Glucose N.D. +0.4 -0.5 -0.0 +0.2
growth on oleic acid YNB Glucose N.D. +0.4 +0.0 -0.2 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.5 -0.1 -0.2 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.2 -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 -0.5 +0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 -1.0 -0.0 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.1 +0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 +0.2 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 +0.1 +0.0 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.1 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.1 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.2 +0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 -0.2 +0.4 +0.2
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