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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11145 and 11146 are separated by 1237 nucleotides
11146 and 11147 are separated by 129 nucleotides
11147 and 11148 are separated by 1083 nucleotides
11148 and 11149 are separated by 173 nucleotides
mRNA_2777: 11145 - K00948 PRPS, prsA ribose-phosphate pyrophosphokinase, at 186,050 to 187,746
11145
mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295
11146
mRNA_2779: 11147 - K18278 THI5 pyrimidine precursor biosynthesis enzyme, at 191,425 to 193,018
11147
mRNA_2780: 11148 - KOG0546 HSP90 co-chaperone CPR7/Cyclophilin, at 194,102 to 194,966
11148
mRNA_2781: 11149 - K09506 DNAJA5 DnaJ homolog subfamily A member 5, at 195,140 to 197,521
11149
Group
Condition
mRNA
_2777
mRNA
_2778
mRNA
_2779
mRNA
_2780
mRNA
_2781
11145
11146
11147
11148
11149
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-1.3
-0.8
-0.4
-0.6
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
-0.8
-0.7
-0.2
-0.4
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
-0.3
-0.5
-0.3
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
-0.4
-0.2
+0.2
-0.2
growth on ypd
YPD
N.D.
-0.2
-0.4
+0.0
-0.1
auxotrophy
Dropout Complete
N.D.
+0.0
-0.2
+0.0
-0.3
auxotrophy
YNB Glucose 75mM Arginine
N.D.
+0.2
-0.3
-0.0
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
+0.3
+0.1
-0.6
-0.2
auxotrophy
YNB Glucose
N.D.
+0.4
-0.5
-0.0
+0.2
growth on oleic acid
YNB Glucose
N.D.
+0.4
+0.0
-0.2
-0.2
growth on ricinoleic acid
YNB Glucose
N.D.
+0.5
-0.1
-0.2
-0.2
growth on oleic acid
YNB Oleic Acid
N.D.
+0.1
+0.2
-0.3
+0.1
auxotrophy
YNB Glucose 75mM Methionine
N.D.
+0.3
-0.5
+0.4
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
+0.7
-1.0
-0.0
+0.5
ypd buoyancy
40 hrs High Buoyancy
N.D.
+0.2
-0.1
+0.0
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
+0.5
+0.2
-0.3
-0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
+0.3
+0.1
+0.0
+0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
-0.2
+0.1
+0.2
+0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
+0.1
+0.1
+0.2
+0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.1
+0.2
+0.1
+0.4
ypd buoyancy
40 hrs Low Buoyancy
N.D.
+0.4
-0.2
+0.4
+0.2
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