Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11148 and 11149 are separated by 173 nucleotides11149 and 11150 are separated by 1052 nucleotides11150 and 11151 are separated by 847 nucleotides11151 and 11152 are separated by 1646 nucleotides mRNA_2780: 11148 - KOG0546 HSP90 co-chaperone CPR7/Cyclophilin, at 194,102 to 194,966 11148 mRNA_2781: 11149 - K09506 DNAJA5 DnaJ homolog subfamily A member 5, at 195,140 to 197,521 11149 mRNA_2782: 11150 - HMMPfam-MYND finger-PF01753,ProSiteProfiles-Zinc finger MYND-type profile.-PS50865,SUPERFAMILY--SSF144232, at 198,574 to 201,487 11150 mRNA_2783: 11151 - BLAST NADH dehydrogenase (ubiquinone) Fe-S protein 5 [C..., at 202,335 to 202,970 11151 mRNA_2784: 11152 - K20241 WDR44, RAB11BP WD repeat-containing protein 44, at 204,617 to 208,124 11152
Group Condition mRNA_2780 mRNA_2781 mRNA_2782 mRNA_2783 mRNA_2784
11148 11149 11150 11151 11152
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.2 -1.2 N.D. -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.4 -2.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.4 -2.4 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 -1.9 N.D. +0.9
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.6 -0.2 N.D. +0.5
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 -0.8 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.2 -0.6 N.D. +0.0
growth on ricinoleic acid YNB Glucose -0.2 -0.2 -1.1 N.D. +1.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.1 -0.2 N.D. +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.0 -0.2 N.D. -0.0
growth on ypd YPD +0.0 -0.1 -0.7 N.D. +0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.5 -0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.2 -0.7 N.D. +0.9
auxotrophy YNB Glucose -0.0 +0.2 -0.7 N.D. +0.9
growth on oleic acid YNB Glucose -0.2 -0.2 -0.1 N.D. +0.9
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.2 +0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.1 -0.7 N.D. +1.0
auxotrophy Dropout Complete +0.0 -0.3 +0.1 N.D. +0.7
growth on oleic acid YNB Oleic Acid -0.3 +0.1 +0.5 N.D. +0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 +1.8 N.D. +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.3 +1.4 N.D. -0.0
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