Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11171 and 11172 are separated by 735 nucleotides11172 and 11173 are separated by 373 nucleotides11173 and 11174 are separated by 168 nucleotides11174 and 11175 are separated by 2199 nucleotides mRNA_2803: 11171 - K01301 NAALAD N-acetylated-alpha-linked acidic dipeptidase, at 253,457 to 256,700 11171 mRNA_2804: 11172 - K10808 RRM2 ribonucleoside-diphosphate reductase subunit M2, at 257,436 to 258,912 11172 mRNA_2805: 11173 - KOG0128 RNA-binding protein SART3 (RRM superfamily), at 259,286 to 263,641 11173 mRNA_2806: 11174 - Hypothetical Protein, at 263,810 to 265,112 11174 mRNA_2807: 11175 - K11485 MTF2, PCL2 polycomb-like protein 2, at 267,312 to 269,571 11175
Group Condition mRNA_2803 mRNA_2804 mRNA_2805 mRNA_2806 mRNA_2807
11171 11172 11173 11174 11175
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -2.3 N.D. -0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.1 N.D. +0.2 -1.6
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.2 N.D. -0.5 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.7 N.D. -0.0 -0.2
growth on ypd YPD -0.5 -0.2 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 N.D. +0.0 -1.1
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 N.D. -0.2 -0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.2 N.D. +0.0 -0.5
auxotrophy YNB Glucose -0.3 -0.0 N.D. -0.3 -0.1
auxotrophy Dropout Complete +0.0 -0.0 N.D. -0.5 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.5 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 N.D. -0.4 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 +0.4 N.D. +0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.2 N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.4 N.D. +0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.1 N.D. +0.0 +0.5
growth on oleic acid YNB Glucose -0.1 +0.3 N.D. +0.1 +0.1
growth on oleic acid YNB Oleic Acid +0.0 -0.2 N.D. +0.2 +0.8
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