Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11176 and 11177 are separated by 434 nucleotides11177 and 11178 are separated by 185 nucleotides11178 and 11179 are separated by 121 nucleotides11179 and 11180 are separated by 682 nucleotides mRNA_2808: 11176 - KOG4539 Uncharacterized conserved protein, at 269,906 to 271,117 11176 mRNA_2809: 11177 - K08596 SENP7 sentrin-specific protease 7, at 271,552 to 278,521 11177 mRNA_2810: 11178 - K00026 MDH2 malate dehydrogenase, at 278,707 to 280,370 11178 mRNA_2811: 11179 - Hypothetical Protein, at 280,492 to 281,442 11179 mRNA_2812: 11180 - Hypothetical Protein, at 282,125 to 283,432 11180
Group Condition mRNA_2808 mRNA_2809 mRNA_2810 mRNA_2811 mRNA_2812
11176 11177 11178 11179 11180
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.1 N.D. -0.8 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.3 N.D. -1.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.3 N.D. -0.5 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.5 N.D. -1.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 +0.2 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.2 N.D. -0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 N.D. -0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.6 -0.1 N.D. -0.6 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.4 N.D. +0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.2 N.D. -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.0 N.D. -0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.4 +0.2 N.D. +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.1 N.D. -0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. -0.4 +0.1
growth on ypd YPD -0.1 +0.4 N.D. -0.0 +0.1
auxotrophy YNB Glucose +0.1 -0.3 N.D. +0.3 +0.4
growth on oleic acid YNB Glucose -0.3 +0.4 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.6 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.4 +0.1 N.D. +0.2 +0.0
auxotrophy Dropout Complete +0.2 -0.2 N.D. +0.6 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.7 N.D. +0.0 -0.0
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