Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11177 and 11178 are separated by 185 nucleotides11178 and 11179 are separated by 121 nucleotides11179 and 11180 are separated by 682 nucleotides11180 and 11181 are separated by 479 nucleotides mRNA_2809: 11177 - K08596 SENP7 sentrin-specific protease 7, at 271,552 to 278,521 11177 mRNA_2810: 11178 - K00026 MDH2 malate dehydrogenase, at 278,707 to 280,370 11178 mRNA_2811: 11179 - Hypothetical Protein, at 280,492 to 281,442 11179 mRNA_2812: 11180 - Hypothetical Protein, at 282,125 to 283,432 11180 mRNA_2813: 11181 - SUPERFAMILY--SSF52047, at 283,912 to 285,183 11181
Group Condition mRNA_2809 mRNA_2810 mRNA_2811 mRNA_2812 mRNA_2813
11177 11178 11179 11180 11181
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -1.2 -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.8 +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.6 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -1.3 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.4 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -0.3 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. -0.5 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.1 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.2 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.1 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. +0.0 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.4 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.2 +0.0 N.D.
auxotrophy YNB Glucose -0.3 N.D. +0.3 +0.4 N.D.
growth on ypd YPD +0.4 N.D. -0.0 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.2 +0.1 N.D.
auxotrophy Dropout Complete -0.2 N.D. +0.6 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. +0.0 -0.0 N.D.
growth on oleic acid YNB Glucose +0.4 N.D. +0.2 +0.3 N.D.
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