Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11185 and 11186 are separated by 685 nucleotides11186 and 11187 are separated by 223 nucleotides11187 and 11188 are separated by 188 nucleotides11188 and 11189 are separated by 1019 nucleotides mRNA_2817: 11185 - KOG2946 Uncharacterized conserved protein, at 290,103 to 291,394 11185 mRNA_2818: 11186 - K02178 BUB1 checkpoint serine/threonine-protein kinase, at 292,080 to 296,876 11186 mRNA_2819: 11187 - HMMPfam-linker histone H1 and H5 family-PF00538,ProSiteProfiles-Linker histone H1/H5 globular (H15) domain profile.-PS51504,SUPERFAMILY--SSF46785, at 297,100 to 297,771 11187 mRNA_2820: 11188 - HMMPfam-2-oxoacid dehydrogenases acyltransferase (catalytic domain)-PF00198,HMMPfam-Biotin-requiring enzyme-PF00364,HMMPfam-e3 binding domain-PF02817,SUPERFAMILY--SSF47005,SUPERFAMILY--SSF51230,SUPERFAMILY--SSF52777, at 297,960 to 299,453 11188 mRNA_2821: 11189 - BLAST DNA polymerase gamma [Kalmanozyma brasiliensis GH..., at 300,473 to 301,385 11189
Group Condition mRNA_2817 mRNA_2818 mRNA_2819 mRNA_2820 mRNA_2821
11185 11186 11187 11188 11189
auxotrophy Dropout Complete -0.4 -0.3 -0.1 -2.3 N.D.
auxotrophy YNB Glucose +0.0 -0.3 +0.2 -2.8 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.6 +0.4 -2.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.9 +0.7 +0.8 -1.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.0 -0.1 -1.5 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.6 +0.2 +0.1 -1.2 N.D.
growth on ricinoleic acid YNB Glucose -0.1 -0.2 +0.5 -1.5 N.D.
growth on ypd YPD -0.1 -0.3 +0.3 -0.9 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.2 +0.2 -1.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -1.4 -0.0 +1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.4 +0.1 -0.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -1.2 +0.4 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.3 +0.8 -1.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.5 -0.1 +0.1 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.0 -0.1 -0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 +0.7 -0.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.5 -0.1 +1.8 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 +0.2 -0.2 N.D.
growth on oleic acid YNB Glucose +0.6 -0.4 +0.4 -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.7 +0.1 +0.2 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.4 +0.5 +0.7 N.D.
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