Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11186 and 11187 are separated by 223 nucleotides11187 and 11188 are separated by 188 nucleotides11188 and 11189 are separated by 1019 nucleotides11189 and 11190 are separated by 343 nucleotides mRNA_2818: 11186 - K02178 BUB1 checkpoint serine/threonine-protein kinase, at 292,080 to 296,876 11186 mRNA_2819: 11187 - HMMPfam-linker histone H1 and H5 family-PF00538,ProSiteProfiles-Linker histone H1/H5 globular (H15) domain profile.-PS51504,SUPERFAMILY--SSF46785, at 297,100 to 297,771 11187 mRNA_2820: 11188 - HMMPfam-2-oxoacid dehydrogenases acyltransferase (catalytic domain)-PF00198,HMMPfam-Biotin-requiring enzyme-PF00364,HMMPfam-e3 binding domain-PF02817,SUPERFAMILY--SSF47005,SUPERFAMILY--SSF51230,SUPERFAMILY--SSF52777, at 297,960 to 299,453 11188 mRNA_2821: 11189 - BLAST DNA polymerase gamma [Kalmanozyma brasiliensis GH..., at 300,473 to 301,385 11189 mRNA_2822: 11190 - SUPERFAMILY--SSF52047, at 301,729 to 303,000 11190
Group Condition mRNA_2818 mRNA_2819 mRNA_2820 mRNA_2821 mRNA_2822
11186 11187 11188 11189 11190
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 +0.4 +0.2 N.D. -1.3
auxotrophy YNB Glucose -0.3 +0.2 -2.8 N.D. +1.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.1 -0.7 N.D. -0.8
auxotrophy Dropout Complete -0.3 -0.1 -2.3 N.D. +1.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.7 -0.9 N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine -0.6 +0.4 -2.2 N.D. +1.1
growth on ricinoleic acid YNB Glucose -0.2 +0.5 -1.5 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.1 -0.6 N.D. +0.0
growth on oleic acid YNB Glucose -0.4 +0.4 -0.1 N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -1.4 -0.0 +1.0 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.8 -1.4 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 -1.1 N.D. +1.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 -1.0 N.D. +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 -0.2 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate -1.5 -0.1 +1.8 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 +0.8 -1.3 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 -1.2 N.D. +1.5
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.1 -1.5 N.D. +2.3
growth on ypd YPD -0.3 +0.3 -0.9 N.D. +1.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.5 +0.7 N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.1 +0.2 -0.0 N.D. +1.3
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