Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11199 and 11200 are separated by 403 nucleotides11200 and 11201 are separated by 629 nucleotides11201 and 11202 are separated by 300 nucleotides11202 and 11203 are separated by 158 nucleotides mRNA_2831: 11199 - HMMPfam-short chain dehydrogenase-PF00106,PRINTS-Glucose/ribitol dehydrogenase family signature-PR00081,SMART--SM00822,SUPERFAMILY--SSF51735, at 318,054 to 319,183 11199 mRNA_2832: 11200 - KOG1611 Predicted short chain-type dehydrogenase, at 319,587 to 320,810 11200 mRNA_2833: 11201 - KOG0254 Predicted transporter (major facilitator superfamily), at 321,440 to 324,159 11201 mRNA_2834: 11202 - K01001 ALG7 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, at 324,460 to 326,344 11202 mRNA_2835: 11203 - K00022 HADH 3-hydroxyacyl-CoA dehydrogenase, at 326,503 to 328,205 11203
Group Condition mRNA_2831 mRNA_2832 mRNA_2833 mRNA_2834 mRNA_2835
11199 11200 11201 11202 11203
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.1 +0.0 N.D. -1.9
growth on oleic acid YNB Oleic Acid +0.1 +0.1 +0.0 N.D. -1.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.5 -0.3 N.D. -1.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 -0.3 -0.2 N.D. -0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 +0.1 +0.2 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.2 +0.1 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 -0.2 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.4 +0.2 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.3 +0.3 N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 -0.1 -0.0 N.D. -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 -0.2 +0.0 N.D. -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 -0.2 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 +0.6 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.3 -0.1 N.D. -0.0
growth on oleic acid YNB Glucose +0.1 +0.3 +0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose +0.0 +0.5 -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.5 -0.3 N.D. +0.1
growth on ypd YPD +0.1 -0.0 +0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.6 +0.7 -0.4 N.D. -0.1
auxotrophy YNB Glucose +0.4 +0.5 -0.4 N.D. +0.2
auxotrophy Dropout Complete +0.9 +0.6 -0.3 N.D. -0.1
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