Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11212 and 11213 are separated by 192 nucleotides11213 and 11214 are separated by 183 nucleotides11214 and 11215 are separated by 192 nucleotides11215 and 11216 are separated by 950 nucleotides mRNA_2844: 11212 - SUPERFAMILY--SSF54427, at 346,316 to 346,839 11212 mRNA_2845: 11213 - K14767 UTP3, SAS10 U3 small nucleolar RNA-associated protein 3, at 347,032 to 349,782 11213 mRNA_2846: 11214 - KOG3100 Uncharacterized conserved protein, at 349,966 to 351,384 11214 mRNA_2847: 11215 - ProSitePatterns-Zinc finger RING-type signature.-PS00518, at 351,577 to 353,493 11215 mRNA_2848: 11216 - HMMPfam-GMC oxidoreductase-PF00732,HMMPfam-GMC oxidoreductase-PF05199,PIRSF--PIRSF000137,ProSitePatterns-GMC oxidoreductases signature 2.-PS00624,SUPERFAMILY--SSF51905,SUPERFAMILY--SSF54373, at 354,444 to 356,897 11216
Group Condition mRNA_2844 mRNA_2845 mRNA_2846 mRNA_2847 mRNA_2848
11212 11213 11214 11215 11216
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.8 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. -1.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 N.D. N.D. -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. N.D. -0.5 +0.2
auxotrophy Dropout Complete +0.1 N.D. N.D. -0.7 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. -0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose -0.4 N.D. N.D. -0.2 +0.2
growth on oleic acid YNB Oleic Acid -0.2 N.D. N.D. -0.1 -0.1
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. N.D. -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. N.D. -0.1 +0.5
growth on ypd YPD +0.3 N.D. N.D. -0.6 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. N.D. +0.0 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. +0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. N.D. -0.0 -0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. +0.5 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 N.D. N.D. -0.0 +0.1
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