Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11213 and 11214 are separated by 183 nucleotides11214 and 11215 are separated by 192 nucleotides11215 and 11216 are separated by 950 nucleotides11216 and 11217 are separated by 1380 nucleotides mRNA_2845: 11213 - K14767 UTP3, SAS10 U3 small nucleolar RNA-associated protein 3, at 347,032 to 349,782 11213 mRNA_2846: 11214 - KOG3100 Uncharacterized conserved protein, at 349,966 to 351,384 11214 mRNA_2847: 11215 - ProSitePatterns-Zinc finger RING-type signature.-PS00518, at 351,577 to 353,493 11215 mRNA_2848: 11216 - HMMPfam-GMC oxidoreductase-PF00732,HMMPfam-GMC oxidoreductase-PF05199,PIRSF--PIRSF000137,ProSitePatterns-GMC oxidoreductases signature 2.-PS00624,SUPERFAMILY--SSF51905,SUPERFAMILY--SSF54373, at 354,444 to 356,897 11216 mRNA_2849: 11217 - K01611 speD, AMD1 S-adenosylmethionine decarboxylase, at 358,278 to 359,885 11217
Group Condition mRNA_2845 mRNA_2846 mRNA_2847 mRNA_2848 mRNA_2849
11213 11214 11215 11216 11217
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -1.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.8 -0.1 N.D.
auxotrophy Dropout Complete N.D. N.D. -0.7 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.5 -0.1 N.D.
growth on ypd YPD N.D. N.D. -0.6 +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.5 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.3 +0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.1 N.D.
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 -0.1 N.D.
auxotrophy YNB Glucose N.D. N.D. -0.2 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.0 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.0 +0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 +0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 +0.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.4 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.5 +0.1 N.D.
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