Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11214 and 11215 are separated by 192 nucleotides11215 and 11216 are separated by 950 nucleotides11216 and 11217 are separated by 1380 nucleotides11217 and 11218 are separated by 300 nucleotides mRNA_2846: 11214 - KOG3100 Uncharacterized conserved protein, at 349,966 to 351,384 11214 mRNA_2847: 11215 - ProSitePatterns-Zinc finger RING-type signature.-PS00518, at 351,577 to 353,493 11215 mRNA_2848: 11216 - HMMPfam-GMC oxidoreductase-PF00732,HMMPfam-GMC oxidoreductase-PF05199,PIRSF--PIRSF000137,ProSitePatterns-GMC oxidoreductases signature 2.-PS00624,SUPERFAMILY--SSF51905,SUPERFAMILY--SSF54373, at 354,444 to 356,897 11216 mRNA_2849: 11217 - K01611 speD, AMD1 S-adenosylmethionine decarboxylase, at 358,278 to 359,885 11217 mRNA_2850: 11218 - SUPERFAMILY--SSF52047, at 360,186 to 362,999 11218
Group Condition mRNA_2846 mRNA_2847 mRNA_2848 mRNA_2849 mRNA_2850
11214 11215 11216 11217 11218
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.1 +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.8 -0.1 N.D. +0.0
growth on ypd YPD N.D. -0.6 +0.3 N.D. -0.2
auxotrophy Dropout Complete N.D. -0.7 -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 N.D. -0.0
growth on oleic acid YNB Glucose N.D. -0.2 -0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 -0.1 N.D. +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 +0.2 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.2 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 -0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 +0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 +0.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 +0.0 N.D. +0.0
auxotrophy YNB Glucose N.D. -0.2 +0.2 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 +0.1 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 +0.5 N.D. +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.0 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.5 +0.1 N.D. +0.0
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