Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11216 and 11217 are separated by 1380 nucleotides11217 and 11218 are separated by 300 nucleotides11218 and 11219 are separated by 401 nucleotides11219 and 11220 are separated by 270 nucleotides mRNA_2848: 11216 - HMMPfam-GMC oxidoreductase-PF00732,HMMPfam-GMC oxidoreductase-PF05199,PIRSF--PIRSF000137,ProSitePatterns-GMC oxidoreductases signature 2.-PS00624,SUPERFAMILY--SSF51905,SUPERFAMILY--SSF54373, at 354,444 to 356,897 11216 mRNA_2849: 11217 - K01611 speD, AMD1 S-adenosylmethionine decarboxylase, at 358,278 to 359,885 11217 mRNA_2850: 11218 - SUPERFAMILY--SSF52047, at 360,186 to 362,999 11218 mRNA_2851: 11219 - SUPERFAMILY--SSF52047, at 363,401 to 364,630 11219 mRNA_2852: 11220 - K01164 POP1 ribonuclease P/MRP protein subunit POP1, at 364,901 to 367,915 11220
Group Condition mRNA_2848 mRNA_2849 mRNA_2850 mRNA_2851 mRNA_2852
11216 11217 11218 11219 11220
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.0 -0.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.4 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.0 -0.3 N.D.
growth on oleic acid YNB Glucose -0.0 N.D. -0.0 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.0 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.0 +0.1 N.D.
growth on ypd YPD +0.3 N.D. -0.2 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.1 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.0 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.4 -0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.1 +0.0 N.D.
auxotrophy Dropout Complete -0.1 N.D. +0.3 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. +0.0 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.2 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.3 -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.0 +0.3 N.D.
auxotrophy YNB Glucose +0.2 N.D. +0.1 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.2 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. +0.0 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. +0.0 +0.7 N.D.
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