Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11237 and 11238 are separated by 223 nucleotides11238 and 11239 are separated by 413 nucleotides11239 and 11240 are separated by 378 nucleotides11240 and 11241 are separated by 195 nucleotides mRNA_2869: 11237 - KOG4170 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase/Peroxisomal 3-ketoacyl-CoA-thiolase, sterol-binding domain and related enzymes, at 407,863 to 408,924 11237 mRNA_2870: 11238 - SUPERFAMILY--SSF64182, at 409,148 to 410,896 11238 mRNA_2871: 11239 - HMMPfam-bZIP transcription factor-PF00170,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 411,310 to 412,108 11239 mRNA_2872: 11240 - HMMPfam-Pyridine nucleotide-disulphide oxidoreductase-PF00070,PRINTS-FAD-dependent pyridine nucleotide reductase signature-PR00368,PRINTS-Pyridine nucleotide disulphide reductase class-I signature-PR00411,SUPERFAMILY--SSF51905, at 412,487 to 414,015 11240 mRNA_2873: 11241 - HMMPfam-Yeast cortical protein KAR9-PF08580,ProSiteProfiles-GAR domain profile.-PS51460,SUPERFAMILY--SSF143575, at 414,211 to 417,159 11241
Group Condition mRNA_2869 mRNA_2870 mRNA_2871 mRNA_2872 mRNA_2873
11237 11238 11239 11240 11241
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.6 -0.1 -0.5 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 -0.3 -0.4 +0.3
auxotrophy Dropout Complete +0.2 -0.5 -0.2 -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 -0.4 +0.1 -0.0
auxotrophy YNB Glucose +0.1 -0.4 -0.2 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.2 -0.3 -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.0 -0.2 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.2 +0.2 +0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.3 +0.5 -0.2 -0.1 -0.0
growth on ypd YPD -0.3 -0.0 +0.1 +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.4 -0.1 +0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 -0.1 +0.1 -0.2
growth on oleic acid YNB Glucose -0.1 -0.1 +0.1 -0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.0 -0.0 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.1 -0.0 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.1 +0.2 +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.6 -0.3 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.2 -0.0 +0.3 +0.3
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.5 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 -0.0 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.2 +0.3 +0.4 +0.3
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