Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11247 and 11248 are separated by 1059 nucleotides11248 and 11249 are separated by 947 nucleotides11249 and 11250 are separated by 736 nucleotides11250 and 11251 are separated by 1166 nucleotides mRNA_2879: 11247 - HMMPfam-Protein of unknown function (DUF3237)-PF11578, at 434,192 to 435,080 11247 mRNA_2880: 11248 - K10531 pvdA L-ornithine N5-oxygenase, at 436,140 to 438,445 11248 mRNA_2881: 11249 - KOG0254 Predicted transporter (major facilitator superfamily), at 439,393 to 442,330 11249 mRNA_2882: 11250 - Hypothetical Protein, at 443,067 to 444,033 11250 mRNA_2883: 11251 - K16675 ZDHHC9_14_18 palmitoyltransferase ZDHHC9/14/18, at 445,200 to 447,494 11251
Group Condition mRNA_2879 mRNA_2880 mRNA_2881 mRNA_2882 mRNA_2883
11247 11248 11249 11250 11251
growth on oleic acid YNB Oleic Acid -0.3 -0.3 -0.2 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.1 -0.5 -0.6 +0.0
growth on ypd YPD -0.0 +0.0 -0.1 -0.1 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.1 +0.3 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 -0.2 -0.6 -0.5 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.1 -0.3 +0.0 -0.0
auxotrophy YNB Glucose -0.1 -0.2 -0.1 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 +0.1 -0.7 -0.7 +0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.2 +0.0 -0.0 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.0 -0.5 -0.3 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.3 +0.2 +0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.3 -0.0 +0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +1.6 -0.2 -1.3 -0.2 +0.8
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.1 +0.2 +0.2 -0.0
auxotrophy Dropout Complete +0.5 -0.1 -0.1 +0.7 -0.3
growth on oleic acid YNB Glucose +0.6 -0.1 -0.2 +0.0 +0.4
growth on ricinoleic acid YNB Glucose +0.9 +0.0 +0.1 -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 +0.1 +0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.8 +0.2 -0.1 +0.3 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 +0.2 +0.2 -0.4 +0.4
ypd buoyancy 40 hrs Low Buoyancy +1.0 +0.2 +0.1 +0.4 +0.1
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