Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11251 and 11252 are separated by 307 nucleotides11252 and 11253 are separated by 148 nucleotides11253 and 11254 are separated by 348 nucleotides11254 and 11255 are separated by 230 nucleotides mRNA_2883: 11251 - K16675 ZDHHC9_14_18 palmitoyltransferase ZDHHC9/14/18, at 445,200 to 447,494 11251 mRNA_2884: 11252 - K10838 XPC xeroderma pigmentosum group C-complementing protein, at 447,802 to 451,973 11252 mRNA_2885: 11253 - K20246 EGT1 L-histidine Nalpha-methyltransferase / hercynylcysteine S-oxide synthase, at 452,122 to 455,835 11253 mRNA_2886: 11254 - Hypothetical Protein, at 456,184 to 458,722 11254 mRNA_2887: 11255 - K07442 TRM61, GCD14 tRNA (adenine57-N1/adenine58-N1)-methyltransferase catalytic subunit, at 458,953 to 460,669 11255
Group Condition mRNA_2883 mRNA_2884 mRNA_2885 mRNA_2886 mRNA_2887
11251 11252 11253 11254 11255
facs on lipid accumulation Sorted No BODIPY Gate +0.6 -1.6 +0.4 -0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.0 -0.4 -0.0 -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -1.2 +0.7 -0.0 N.D.
growth on ypd YPD -0.5 +0.4 +0.2 -0.2 N.D.
auxotrophy Dropout Complete -0.3 -0.1 +0.0 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 +0.0 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.0 +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.1 -0.1 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.0 +0.2 +0.1 N.D.
auxotrophy YNB Glucose +0.0 +0.1 +0.1 -0.1 N.D.
growth on oleic acid YNB Glucose +0.4 -0.2 +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.3 -0.1 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.0 +0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.1 -0.1 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 +0.1 +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.1 +0.1 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -0.4 +0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 +0.3 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.1 +0.1 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 +0.4 +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.9 +1.6 -0.0 N.D.
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