Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11253 and 11254 are separated by 348 nucleotides11254 and 11255 are separated by 230 nucleotides11255 and 11256 are separated by 451 nucleotides11256 and 11257 are separated by 363 nucleotides mRNA_2885: 11253 - K20246 EGT1 L-histidine Nalpha-methyltransferase / hercynylcysteine S-oxide synthase, at 452,122 to 455,835 11253 mRNA_2886: 11254 - Hypothetical Protein, at 456,184 to 458,722 11254 mRNA_2887: 11255 - K07442 TRM61, GCD14 tRNA (adenine57-N1/adenine58-N1)-methyltransferase catalytic subunit, at 458,953 to 460,669 11255 mRNA_2888: 11256 - KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein vp115/Granuphilin A, contains C2 domain, at 461,121 to 465,686 11256 mRNA_2889: 11257 - K08184 SLC16A7 MFS transporter, MCP family, solute carrier family 16 (monocarboxylic acid transporters), member 7, at 466,050 to 468,099 11257
Group Condition mRNA_2885 mRNA_2886 mRNA_2887 mRNA_2888 mRNA_2889
11253 11254 11255 11256 11257
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +0.1 N.D. -0.1 -0.3
growth on ypd YPD +0.2 -0.2 N.D. +0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 N.D. -0.1 -0.3
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. -0.0 -0.1
growth on ricinoleic acid YNB Glucose +0.1 -0.1 N.D. -0.1 -0.0
auxotrophy YNB Glucose +0.1 -0.1 N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid -0.0 -0.1 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.0 N.D. -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.3 N.D. +0.1 -0.3
auxotrophy Dropout Complete +0.0 +0.2 N.D. +0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.3 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.3 N.D. -0.0 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.7 -0.0 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.0 N.D. +0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.2 N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.3 N.D. +0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 N.D. +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 N.D. +0.3 +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 N.D. +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +1.6 -0.0 N.D. +0.1 -0.5
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