Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11297 and 11298 are separated by 475 nucleotides11298 and 11299 are separated by 313 nucleotides11299 and 11300 are separated by 232 nucleotides11300 and 11301 are separated by 159 nucleotides mRNA_2929: 11297 - Hypothetical Protein, at 576,993 to 577,700 11297 mRNA_2930: 11298 - Interpro Nbl1 / Borealin N terminal, at 578,176 to 578,978 11298 mRNA_2931: 11299 - K06676 BRRN1, BRN1, CAPH condensin complex subunit 2, at 579,292 to 582,332 11299 mRNA_2932: 11300 - Hypothetical Protein, at 582,565 to 583,032 11300 mRNA_2933: 11301 - Hypothetical Protein, at 583,192 to 584,593 11301
Group Condition mRNA_2929 mRNA_2930 mRNA_2931 mRNA_2932 mRNA_2933
11297 11298 11299 11300 11301
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.4 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 -0.1
auxotrophy YNB Glucose N.D. N.D. N.D. -0.6 +0.2
auxotrophy Dropout Complete N.D. N.D. N.D. -0.4 +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.0 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.1 +0.2
growth on ypd YPD N.D. N.D. N.D. +0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. +0.3 -0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.3 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.2 +0.4
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