Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11311 and 11312 are separated by 1055 nucleotides11312 and 11313 are separated by 257 nucleotides11313 and 11314 are separated by 524 nucleotides11314 and 11315 are separated by 869 nucleotides mRNA_2943: 11311 - KOG1902 Putative signal transduction protein involved in RNA splicing, at 609,687 to 613,545 11311 mRNA_2944: 11312 - BLAST Putative CigA protein [Rhizopus microsporus], at 614,601 to 617,050 11312 mRNA_2945: 11313 - K15175 CDC73 parafibromin, at 617,308 to 618,975 11313 mRNA_2946: 11314 - SUPERFAMILY--SSF56112, at 619,500 to 621,824 11314 mRNA_2947: 11315 - Hypothetical Protein, at 622,694 to 625,091 11315
Group Condition mRNA_2943 mRNA_2944 mRNA_2945 mRNA_2946 mRNA_2947
11311 11312 11313 11314 11315
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. -0.5 -0.2
growth on ypd YPD +0.0 -0.3 N.D. +0.0 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.4 N.D. +0.1 +0.4
growth on oleic acid YNB Glucose +0.2 -0.0 N.D. -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.3 N.D. +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.3 N.D. +0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 N.D. -0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 N.D. -0.2 -0.0
auxotrophy YNB Glucose +0.1 +0.3 N.D. -0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.2 N.D. -0.2 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.4 N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.0 N.D. +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.2 N.D. -0.0 +0.3
auxotrophy Dropout Complete -0.1 +0.8 N.D. +0.0 -0.2
growth on ricinoleic acid YNB Glucose +0.2 +0.4 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.8 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.8 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.8 N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.9 N.D. +0.2 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +1.7 N.D. +0.2 +0.2
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