Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11326 and 11327 are separated by 1087 nucleotides11327 and 11328 are separated by 411 nucleotides11328 and 11329 are separated by 990 nucleotides11329 and 11330 are separated by 444 nucleotides mRNA_2958: 11326 - K17907 ATG9 autophagy-related protein 9, at 647,495 to 650,461 11326 mRNA_2959: 11327 - KOG4554 Protein involved in inorganic phosphate transport, at 651,549 to 652,747 11327 mRNA_2960: 11328 - KOG2617 Citrate synthase, at 653,159 to 654,002 11328 mRNA_2961: 11329 - ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 654,993 to 657,914 11329 mRNA_2962: 11330 - KOG3841 TEF-1 and related transcription factor, TEAD family, at 658,359 to 659,860 11330
Group Condition mRNA_2958 mRNA_2959 mRNA_2960 mRNA_2961 mRNA_2962
11326 11327 11328 11329 11330
auxotrophy Dropout Complete -0.2 N.D. -0.8 -0.7 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -1.2 N.D. -0.5 -0.4 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.1 -1.0 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.8 N.D. +0.5 -1.1 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.3 -0.3 -0.5
auxotrophy YNB Glucose -0.0 N.D. -0.0 -0.5 -0.7
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. -0.1 -0.5 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 N.D. +0.6 -0.8 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -1.0 N.D. -0.3 -0.1 +0.3
growth on oleic acid YNB Oleic Acid +0.2 N.D. -0.3 -0.3 -0.6
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.3 -0.4 -0.2
growth on oleic acid YNB Glucose -0.1 N.D. -0.2 -0.0 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. +0.2 -0.6 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.5 -0.7 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.1 -0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.3 -0.7 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. +1.0 -0.9 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.4 -0.2 -0.2
growth on ypd YPD -0.1 N.D. +0.5 +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.8 +0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +1.5 +0.5 +0.1
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