Fitness for 3 genes in Rhodosporidium toruloides IFO0880

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500 nt 11348 and 11349 are separated by 2046 nucleotides11349 and 11350 are separated by 2173 nucleotides mRNA_2980: 11348 - KOG3140 Predicted membrane protein, at 23 to 1,444 11348 mRNA_2981: 11349 - SUPERFAMILY--SSF50685, at 3,491 to 5,123 11349 mRNA_2982: 11350 - Hypothetical Protein, at 7,297 to 8,646 11350
Group Condition mRNA_2980 mRNA_2981 mRNA_2982
11348 11349 11350
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 -0.1
auxotrophy YNB Glucose +0.3 -0.0 -0.8
growth on ricinoleic acid YNB Glucose -0.1 +0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.0 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 -0.0
auxotrophy Dropout Complete +0.4 +0.2 -0.6
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.4 -0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.0 +0.1
growth on oleic acid YNB Oleic Acid +0.4 +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.6 +0.2 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 +0.5
growth on ypd YPD +0.2 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 -0.3
growth on oleic acid YNB Glucose +0.4 +0.3 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.4 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.0 +0.6
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