Fitness for 4 genes in Rhodosporidium toruloides IFO0880

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500 nt 11348 and 11349 are separated by 2046 nucleotides11349 and 11350 are separated by 2173 nucleotides11350 and 11351 are separated by 244 nucleotides mRNA_2980: 11348 - KOG3140 Predicted membrane protein, at 23 to 1,444 11348 mRNA_2981: 11349 - SUPERFAMILY--SSF50685, at 3,491 to 5,123 11349 mRNA_2982: 11350 - Hypothetical Protein, at 7,297 to 8,646 11350 mRNA_2983: 11351 - K00993 EPT1 ethanolaminephosphotransferase, at 8,891 to 11,294 11351
Group Condition mRNA_2980 mRNA_2981 mRNA_2982 mRNA_2983
11348 11349 11350 11351
auxotrophy YNB Glucose +0.3 -0.0 -0.8 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 -0.1 +0.1
growth on ricinoleic acid YNB Glucose -0.1 +0.0 -0.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.0 -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 -0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 -0.0 -0.0
auxotrophy Dropout Complete +0.4 +0.2 -0.6 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.4 -0.7 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.6 +0.2 -0.6 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.3 -0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.4 +0.1 -0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 -0.5 +0.3
growth on ypd YPD +0.2 -0.1 +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.0 -0.1 +0.1
growth on oleic acid YNB Glucose +0.4 +0.3 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 +0.5 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.0 +0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.4 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.0 +0.6 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.2 +0.3 +0.5
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