Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11363 and 11364 are separated by 115 nucleotides11364 and 11365 are separated by 173 nucleotides11365 and 11366 are separated by 182 nucleotides11366 and 11367 are separated by 376 nucleotides mRNA_2995: 11363 - K20290 COG3, SEC34 conserved oligomeric Golgi complex subunit 3, at 34,550 to 37,704 11363 mRNA_2996: 11364 - Hypothetical Protein, at 37,820 to 39,043 11364 mRNA_2997: 11365 - K14768 UTP7, WDR46 U3 small nucleolar RNA-associated protein 7, at 39,217 to 41,331 11365 mRNA_2998: 11366 - KOG3383 Uncharacterized conserved protein, at 41,514 to 42,818 11366 mRNA_2999: 11367 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 43,195 to 45,486 11367
Group Condition mRNA_2995 mRNA_2996 mRNA_2997 mRNA_2998 mRNA_2999
11363 11364 11365 11366 11367
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. -0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. N.D. -0.2 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. -0.3 +0.2
growth on ypd YPD +0.1 N.D. N.D. +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. -0.4 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.6 +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 N.D. N.D. -0.7 +0.0
growth on oleic acid YNB Glucose +0.4 N.D. N.D. -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. -0.0 +0.1
growth on ricinoleic acid YNB Glucose +0.5 N.D. N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. +0.5 +0.1
growth on oleic acid YNB Oleic Acid +0.4 N.D. N.D. +0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. N.D. -0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +1.1 N.D. N.D. -0.4 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. +0.6 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.8 N.D. N.D. -0.0 +0.4
auxotrophy Dropout Complete +0.6 N.D. N.D. -0.0 +0.5
auxotrophy YNB Glucose +0.9 N.D. N.D. -0.2 +0.5
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