Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11369 and 11370 are separated by 43 nucleotides11370 and 11371 are separated by 586 nucleotides11371 and 11372 are separated by 166 nucleotides11372 and 11373 are separated by 630 nucleotides mRNA_3001: 11369 - ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 48,202 to 49,332 11369 mRNA_3002: 11370 - HMMPfam-Fungal specific transcription factor domain-PF11951, at 49,376 to 50,038 11370 mRNA_3003: 11371 - ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SUPERFAMILY--SSF57701, at 50,625 to 53,414 11371 mRNA_3004: 11372 - K12624 LSM5 U6 snRNA-associated Sm-like protein LSm5, at 53,581 to 54,123 11372 mRNA_3005: 11373 - K19708 PTC6, AUP1 protein phosphatase PTC6, at 54,754 to 56,074 11373
Group Condition mRNA_3001 mRNA_3002 mRNA_3003 mRNA_3004 mRNA_3005
11369 11370 11371 11372 11373
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.7 -0.7 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.5 -0.1 N.D. -2.2
growth on ypd YPD -0.4 -0.6 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.1 +0.1 N.D. -0.5
growth on oleic acid YNB Oleic Acid +0.2 -0.3 +0.1 N.D. -0.8
growth on oleic acid YNB Glucose +0.1 +0.1 -0.1 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.1 -0.1 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 -0.2 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 +0.1 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 +0.1 N.D. -0.3
auxotrophy Dropout Complete +0.2 -0.3 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.2 +0.3 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.0 -0.1 +0.1 N.D. -0.1
auxotrophy YNB Glucose +0.2 -0.2 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 +0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.1 -0.1 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.4 +0.1 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 +0.0 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.5 +0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.3 +0.1 N.D. +0.1
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