Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11387 and 11388 are separated by 2550 nucleotides11388 and 11389 are separated by 324 nucleotides11389 and 11390 are separated by 355 nucleotides11390 and 11391 are separated by 252 nucleotides mRNA_3019: 11387 - KOG2128 Ras GTPase-activating protein family - IQGAP, at 111,732 to 112,142 11387 mRNA_3020: 11388 - Hypothetical Protein, at 114,693 to 115,018 11388 mRNA_3021: 11389 - K00697 otsA trehalose 6-phosphate synthase, at 115,343 to 117,642 11389 mRNA_3022: 11390 - K12394 AP1S1_2 AP-1 complex subunit sigma 1/2, at 117,998 to 118,989 11390 mRNA_3023: 11391 - K04709 LAG1 Acyl-CoA-dependent ceramide synthase, at 119,242 to 120,921 11391
Group Condition mRNA_3019 mRNA_3020 mRNA_3021 mRNA_3022 mRNA_3023
11387 11388 11389 11390 11391
growth on ypd YPD -2.4 N.D. -0.4 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.8 N.D. -0.9 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. -0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.3 N.D. +0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. -0.3 N.D. N.D.
auxotrophy Dropout Complete -0.4 N.D. -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.9 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.4 N.D. N.D.
auxotrophy YNB Glucose -0.0 N.D. -0.3 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.2 N.D. +0.9 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.5 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. +0.3 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid -1.5 N.D. +1.6 N.D. N.D.
growth on oleic acid YNB Glucose +0.1 N.D. +0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 N.D. -0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.4 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +2.2 N.D. -0.9 N.D. N.D.
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