Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11388 and 11389 are separated by 324 nucleotides11389 and 11390 are separated by 355 nucleotides11390 and 11391 are separated by 252 nucleotides11391 and 11392 are separated by 155 nucleotides mRNA_3020: 11388 - Hypothetical Protein, at 114,693 to 115,018 11388 mRNA_3021: 11389 - K00697 otsA trehalose 6-phosphate synthase, at 115,343 to 117,642 11389 mRNA_3022: 11390 - K12394 AP1S1_2 AP-1 complex subunit sigma 1/2, at 117,998 to 118,989 11390 mRNA_3023: 11391 - K04709 LAG1 Acyl-CoA-dependent ceramide synthase, at 119,242 to 120,921 11391 mRNA_3024: 11392 - K20366 ERGIC2, ERV41 endoplasmic reticulum-Golgi intermediate compartment protein 2, at 121,077 to 122,686 11392
Group Condition mRNA_3020 mRNA_3021 mRNA_3022 mRNA_3023 mRNA_3024
11388 11389 11390 11391 11392
growth on ricinoleic acid YNB Glucose N.D. -0.9 N.D. N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.9 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.9 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.6 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. N.D. -0.2
growth on ypd YPD N.D. -0.4 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. N.D. -0.0
auxotrophy YNB Glucose N.D. -0.3 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 N.D. N.D. +0.2
auxotrophy Dropout Complete N.D. -0.1 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. N.D. +0.6
growth on oleic acid YNB Glucose N.D. +0.3 N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 N.D. N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 N.D. N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.9 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. +1.6 N.D. N.D. -0.0
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