Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11390 and 11391 are separated by 252 nucleotides11391 and 11392 are separated by 155 nucleotides11392 and 11393 are separated by 520 nucleotides11393 and 11394 are separated by 664 nucleotides mRNA_3022: 11390 - K12394 AP1S1_2 AP-1 complex subunit sigma 1/2, at 117,998 to 118,989 11390 mRNA_3023: 11391 - K04709 LAG1 Acyl-CoA-dependent ceramide synthase, at 119,242 to 120,921 11391 mRNA_3024: 11392 - K20366 ERGIC2, ERV41 endoplasmic reticulum-Golgi intermediate compartment protein 2, at 121,077 to 122,686 11392 mRNA_3025: 11393 - K11354 cph1 two-component system, chemotaxis family, sensor kinase Cph1, at 123,207 to 127,605 11393 mRNA_3026: 11394 - Hypothetical Protein, at 128,270 to 129,253 11394
Group Condition mRNA_3022 mRNA_3023 mRNA_3024 mRNA_3025 mRNA_3026
11390 11391 11392 11393 11394
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 +0.0 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 -0.0 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.3 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 +0.2 -0.3
auxotrophy YNB Glucose N.D. N.D. +0.1 -0.1 -0.2
growth on ypd YPD N.D. N.D. -0.2 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.2 -0.2
growth on oleic acid YNB Glucose N.D. N.D. +0.1 +0.1 -0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.0 -0.0 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 +0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 +0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.0 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.1 -0.1
auxotrophy Dropout Complete N.D. N.D. +0.0 -0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.2 +0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.6 -0.1 -0.1
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