Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11416 and 11417 are separated by 285 nucleotides11417 and 11418 are separated by 374 nucleotides11418 and 11419 are separated by 667 nucleotides11419 and 11420 are separated by 494 nucleotides mRNA_3048: 11416 - K03514 PAPD5_7, TRF4 non-canonical poly(A) RNA polymerase PAPD5/7, at 188,658 to 190,471 11416 mRNA_3049: 11417 - SUPERFAMILY--SSF50685, at 190,757 to 193,605 11417 mRNA_3050: 11418 - K14430 PHO87_91 phosphate transporter, at 193,980 to 198,261 11418 mRNA_3051: 11419 - Hypothetical Protein, at 198,929 to 201,085 11419 mRNA_3052: 11420 - KOG2404 Fumarate reductase, flavoprotein subunit, at 201,580 to 204,661 11420
Group Condition mRNA_3048 mRNA_3049 mRNA_3050 mRNA_3051 mRNA_3052
11416 11417 11418 11419 11420
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. -0.7 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. -0.9 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.7 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. N.D. +0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.0 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. -0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. +0.2 -0.1
auxotrophy YNB Glucose -0.0 N.D. N.D. +0.3 -0.2
growth on oleic acid YNB Glucose +0.0 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.0 +0.1
auxotrophy Dropout Complete -0.0 N.D. N.D. +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. +0.5 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +0.7 -0.3
growth on ricinoleic acid YNB Glucose -0.0 N.D. N.D. +0.4 +0.0
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. +0.6 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.6 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. +0.4 -0.1
growth on ypd YPD +0.1 N.D. N.D. +0.7 -0.2
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