Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11434 and 11435 are separated by 1813 nucleotides11435 and 11436 are separated by 303 nucleotides11436 and 11437 are separated by 215 nucleotides11437 and 11438 are separated by 234 nucleotides mRNA_3066: 11434 - K13449 PR1 pathogenesis-related protein 1, at 232,396 to 233,465 11434 mRNA_3067: 11435 - HMMPfam-Taurine catabolism dioxygenase TauD, TfdA family-PF02668,SUPERFAMILY--SSF51197, at 235,279 to 236,676 11435 mRNA_3068: 11436 - K02434 gatB, PET112 aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit B, at 236,980 to 239,400 11436 mRNA_3069: 11437 - KOG2505 Ankyrin repeat protein, at 239,616 to 241,978 11437 mRNA_3070: 11438 - Hypothetical Protein, at 242,213 to 243,874 11438
Group Condition mRNA_3066 mRNA_3067 mRNA_3068 mRNA_3069 mRNA_3070
11434 11435 11436 11437 11438
growth on ypd YPD -0.2 -0.1 -0.5 -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.2 -0.0 -0.3 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.1 -0.2 -0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.4 +0.4 -0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 +0.0 +0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 -0.0 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 +0.0 -0.2 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.2 -0.0 -0.1 +0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.1 +0.2 +0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 -0.0 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.1 -0.1 -0.2 +0.2
growth on oleic acid YNB Glucose +0.1 -0.1 +0.1 +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.2 -0.2 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.1 -0.1 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 -0.1 -0.2 -0.1 +0.3
growth on oleic acid YNB Oleic Acid +0.3 -0.0 +0.1 +0.2 +0.1
auxotrophy Dropout Complete -0.0 +0.0 +0.2 +0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.0 +0.1 +0.1 +0.3
auxotrophy YNB Glucose +0.3 +0.1 +0.1 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 -0.1 -0.1 -0.1 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.0 +0.2 +0.2 +0.2
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