Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11436 and 11437 are separated by 215 nucleotides11437 and 11438 are separated by 234 nucleotides11438 and 11439 are separated by 1703 nucleotides11439 and 11440 are separated by 976 nucleotides mRNA_3068: 11436 - K02434 gatB, PET112 aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit B, at 236,980 to 239,400 11436 mRNA_3069: 11437 - KOG2505 Ankyrin repeat protein, at 239,616 to 241,978 11437 mRNA_3070: 11438 - Hypothetical Protein, at 242,213 to 243,874 11438 mRNA_3071: 11439 - Hypothetical Protein, at 245,578 to 247,225 11439 mRNA_3072: 11440 - KOG1203 Predicted dehydrogenase, at 248,202 to 249,247 11440
Group Condition mRNA_3068 mRNA_3069 mRNA_3070 mRNA_3071 mRNA_3072
11436 11437 11438 11439 11440
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.1 -0.4 -0.4 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.3 +0.1 -0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +0.1 -0.3 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.2 +0.2 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.3 -0.1 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.0 +0.1 -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.2 -0.0 +0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.1 -0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 +0.3 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.3 -0.0 +0.1 +0.0
growth on ypd YPD -0.5 -0.0 +0.1 +0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.2 +0.0 -0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 +0.3 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 +0.5 -0.1 +0.1
growth on oleic acid YNB Glucose +0.1 +0.1 +0.1 +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.1 +0.4 +0.3 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 +0.3 +0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.1 +0.2 +0.1 +0.1 +0.2
auxotrophy YNB Glucose +0.1 +0.2 +0.3 +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 +0.2 +0.1 +0.1
auxotrophy Dropout Complete +0.2 +0.2 +0.3 -0.0 +0.2
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