Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11440 and 11441 are separated by 153 nucleotides11441 and 11442 are separated by 45 nucleotides11442 and 11443 are separated by 218 nucleotides11443 and 11444 are separated by 1326 nucleotides mRNA_3072: 11440 - KOG1203 Predicted dehydrogenase, at 248,202 to 249,247 11440 mRNA_3073: 11441 - ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,SUPERFAMILY--SSF57667, at 249,401 to 250,160 11441 mRNA_3074: 11442 - KOG4650 Predicted steroid reductase, at 250,206 to 251,968 11442 mRNA_3075: 11443 - K07652 vicK two-component system, OmpR family, sensor histidine kinase VicK, at 252,187 to 260,484 11443 mRNA_3076: 11444 - KOG4497 Uncharacterized conserved protein WDR8, contains WD repeats, at 261,811 to 264,179 11444
Group Condition mRNA_3072 mRNA_3073 mRNA_3074 mRNA_3075 mRNA_3076
11440 11441 11442 11443 11444
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.4 +0.2 -0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.2 -0.0 +0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 +0.3 +0.0 -0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.4 -0.0 +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 -0.1 -0.0 +0.2
growth on oleic acid YNB Glucose +0.2 -0.2 +0.1 +0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.2 -0.0 +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.0 +0.1 +0.0 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.1 +0.0 +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 +0.1 +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.0 +0.2 +0.2 -0.0
growth on ricinoleic acid YNB Glucose -0.1 +0.1 +0.1 +0.2 +0.0
auxotrophy Dropout Complete +0.2 -0.1 +0.1 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.0 +0.0 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 +0.2 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.0 +0.5 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 +0.2 +0.1 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.1 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 +0.3 +0.1 +0.3
auxotrophy YNB Glucose +0.1 -0.0 +0.3 +0.1 +0.2
growth on ypd YPD +0.3 +0.1 +0.2 +0.1 +0.1
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