Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11441 and 11442 are separated by 45 nucleotides11442 and 11443 are separated by 218 nucleotides11443 and 11444 are separated by 1326 nucleotides11444 and 11445 are separated by 29 nucleotides mRNA_3073: 11441 - ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,SUPERFAMILY--SSF57667, at 249,401 to 250,160 11441 mRNA_3074: 11442 - KOG4650 Predicted steroid reductase, at 250,206 to 251,968 11442 mRNA_3075: 11443 - K07652 vicK two-component system, OmpR family, sensor histidine kinase VicK, at 252,187 to 260,484 11443 mRNA_3076: 11444 - KOG4497 Uncharacterized conserved protein WDR8, contains WD repeats, at 261,811 to 264,179 11444 mRNA_3077: 11445 - K07542 PIGV phosphatidylinositol glycan, class V, at 264,209 to 265,729 11445
Group Condition mRNA_3073 mRNA_3074 mRNA_3075 mRNA_3076 mRNA_3077
11441 11442 11443 11444 11445
growth on oleic acid YNB Oleic Acid -0.4 -0.0 +0.1 -0.0 +0.0
growth on oleic acid YNB Glucose -0.2 +0.1 +0.0 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.3 +0.0 -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 -0.0 +0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.2 -0.1 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.0 +0.2 -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 +0.2 +0.0 -0.1
auxotrophy Dropout Complete -0.1 +0.1 +0.2 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.1 +0.0 +0.0 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.0 +0.1 -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.2 +0.2 -0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 -0.0 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.3 +0.1 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.2 +0.0 +0.3 -0.1
growth on ypd YPD +0.1 +0.2 +0.1 +0.1 -0.0
growth on ricinoleic acid YNB Glucose +0.1 +0.1 +0.2 +0.0 +0.1
auxotrophy YNB Glucose -0.0 +0.3 +0.1 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.2 +0.1 +0.5 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.3 +0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.0 +0.1 +0.3 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.5 +0.1 +0.2 +0.1
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