Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11442 and 11443 are separated by 218 nucleotides11443 and 11444 are separated by 1326 nucleotides11444 and 11445 are separated by 29 nucleotides11445 and 11446 are separated by 175 nucleotides mRNA_3074: 11442 - KOG4650 Predicted steroid reductase, at 250,206 to 251,968 11442 mRNA_3075: 11443 - K07652 vicK two-component system, OmpR family, sensor histidine kinase VicK, at 252,187 to 260,484 11443 mRNA_3076: 11444 - KOG4497 Uncharacterized conserved protein WDR8, contains WD repeats, at 261,811 to 264,179 11444 mRNA_3077: 11445 - K07542 PIGV phosphatidylinositol glycan, class V, at 264,209 to 265,729 11445 mRNA_3078: 11446 - K01613 psd, PISD phosphatidylserine decarboxylase, at 265,905 to 270,528 11446
Group Condition mRNA_3074 mRNA_3075 mRNA_3076 mRNA_3077 mRNA_3078
11442 11443 11444 11445 11446
growth on oleic acid YNB Glucose +0.1 +0.0 -0.2 -0.0 +0.0
growth on oleic acid YNB Oleic Acid -0.0 +0.1 -0.0 +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 +0.2 +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.2 -0.1 -0.2 +0.2
auxotrophy Dropout Complete +0.1 +0.2 +0.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 +0.0 -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 +0.0 +0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.2 -0.0 -0.0 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 +0.3 -0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.2 +0.0 +0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.1 -0.0 +0.1 +0.2
auxotrophy YNB Glucose +0.3 +0.1 +0.2 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.0 -0.1 +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.0 +0.2 +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.3 +0.1 -0.0 +0.0
growth on ypd YPD +0.2 +0.1 +0.1 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.1 +0.3 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 +0.2 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.1 +0.3 +0.5 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 +0.1 +0.2 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.1 +0.5 +0.0 +0.3
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