Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11443 and 11444 are separated by 1326 nucleotides11444 and 11445 are separated by 29 nucleotides11445 and 11446 are separated by 175 nucleotides11446 and 11447 are separated by 384 nucleotides mRNA_3075: 11443 - K07652 vicK two-component system, OmpR family, sensor histidine kinase VicK, at 252,187 to 260,484 11443 mRNA_3076: 11444 - KOG4497 Uncharacterized conserved protein WDR8, contains WD repeats, at 261,811 to 264,179 11444 mRNA_3077: 11445 - K07542 PIGV phosphatidylinositol glycan, class V, at 264,209 to 265,729 11445 mRNA_3078: 11446 - K01613 psd, PISD phosphatidylserine decarboxylase, at 265,905 to 270,528 11446 mRNA_3079: 11447 - HMMPfam-DNA polymerase Ligase (LigD)-PF13298, at 270,913 to 272,289 11447
Group Condition mRNA_3075 mRNA_3076 mRNA_3077 mRNA_3078 mRNA_3079
11443 11444 11445 11446 11447
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.1 -0.2 +0.2 -0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.1 +0.0 +0.2 -0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.0 +0.0 -0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.0 +0.2 -0.0 -0.2
growth on oleic acid YNB Glucose +0.0 -0.2 -0.0 +0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.1 -0.0 +0.0 -0.3
growth on ricinoleic acid YNB Glucose +0.2 +0.0 +0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 -0.1 -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 +0.0 -0.1 +0.2
auxotrophy Dropout Complete +0.2 +0.1 -0.1 -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.0 -0.0 -0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.0 -0.1 -0.1 +0.2
auxotrophy YNB Glucose +0.1 +0.2 -0.1 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.3 -0.1 -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.2 +0.1 +0.2 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.0 +0.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.2 +0.1 +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 +0.1 +0.1 +0.2
growth on ypd YPD +0.1 +0.1 -0.0 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.5 +0.0 +0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.3 +0.5 +0.0 +0.1
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